開催日

• 2025/11/6

  10:30〜11:30

開催場所（方法）

京都大学アイセムス本館 2階 セミナールーム（A207)

詳細

Native mass spectrometry for probing membrane protein dynamics

Understanding protein–protein and protein–ligand interactions is essential for elucidating the biological functions of proteins. However, gathering such information is challenging and complex. Native mass spectrometry (native-MS) has emerged as a powerful analytical approach for protein characterization under non-denaturing conditions. By preserving noncovalent interactions in the gas phase, native-MS enables robust and detailed investigation of complex biomolecular assemblies. In this talk, I will highlight the application of native-MS to address key questions in membrane biology, including the membrane interactome, protein–lipid dynamics, and glycosylation. Recent advances in methodology and instrumentation development, such as charge-detection mass spectrometry, proton-transfer-reaction mass spectrometry and native top-down mass spectrometry, will be introduced to further expand the scope of native-MS applications. Collectively, these developments underscore the role of mass spectrometry as a central technique for probing the dynamics of integral membrane proteins in their native environment, particularly in complement to the recent breakthroughs achieved with cryoelectron microscopy.

申し込み

不要

参加費

無料/Free of charge

お問い合わせ

京都大学 高等研究院 アイセムス 鈴木淳
jsuzuki@icems.kyoto-u.ac.jp