Microbiology and Infectious Diseases

We are studying about the infectious diseases that are caused by a virus among the microbes that cause illness to humans. Unlike other microbes, such as bacteria and fungus, multiplication of a virus is achieved by using a part of function of a host cell. Therefore, the coexistence of a virus with a host cell is indispensable. Our research focuses on the mechanisms of entry, replication, immune evasion, and pathogenesis of HIV-1 that is a cause of the acquired immunodeficiency syndrome (AIDS).

Research and Education

In our laboratory, we analyze the interaction between virus and host (host cell), and we reveal both the immune host defense mechanisms and viral pathogenicity. Currently, we are doing research as a core theme of the next two.

1) Elucidation of the cell invasion mechanism of the human immunodeficiency virus (HIV-1).

A dendritic cell (DC) and some macrophage-like cells have the DC-SIGN molecules which are one of the C type lectin receptors which can be connected in HIV-1 by the cohesion that is stronger than CD4 molecules. We pay attention to the endosomal pathway for viral expansion and analyze events in this receptor and cell after the bond of the virus particle (.Fig 1.).

2) Analysis of the protective immunity and the prevention of onset diseases against HIV-1.

Using simian immunodeficiency virus (SIV) and the immunedeficient mouse which transplanted the hematopoietic stem cell, we would like to clarify the causes, biological and prevention of disease of HIV-1 infection (Fig 2.).

Pathway of endocytosis.

Pathway of endocytosis.


Purification of CD3-CD34+CD38- cells by magnetic beads from rhesus bone marrow.

Purification of CD3-CD34+CD38- cells by magnetic beads from rhesus bone marrow.


Publications

1. Himeno A, Akagi T, Uto T, Wang X, Baba M, Ibuki K et al. (2010) Evaluation of the immune response and protective effects of rhesus macaques vaccinated with biodegradable nanoparticles carrying gp120 of human immunodeficiency virus. Vaccine 28(32) 5377-5385.
2. Matsuda K, Inaba K, Fukazawa Y, Matsuyama M, Ibuki K et al. (2010) In vivo analysis of a new R5 tropic SHIV generated from the highly pathogenic SHIV-KS661, a derivative of SHIV-89.6. Virology 399(1) 134-143.
3. Inaba K, Fukazawa Y, Matsuda K, Himeno A, Matsuyama M, Ibuki K et al. (2010) Small intestine CD4+ cell reduction and enteropathy in simian/human immunodeficiency virus KS661-infected rhesus macaques in the presence of low viral load. J.Gen.Virol. 91(Pt3) 773-781.
4. Yamamoto H, Li TC, Koshimoto C, Ito K, Kita M, Miyashita N, Arikawa J, Yagami K, Asano M, Tezuka H, Suzuki N, Kurosawa T, Shibahara T, Furuya M, Mohri S, Sato H, Ohsawa K, Ibuki K, Takeda N. (2008) Serological evidence for hepatitis E virus infection in laboratory monkeys and pigs in animal facilities in Japan. Exp. Anim. 57(4) 367-376.
5. Fukazawa Y, Miyake A, Ibuki K et al. (2008) Small intestine CD4+ T cells are profoundly depleted during acute simian-human immunodeficiency virus infection, regardless of viral pathogenicity. J.Virol. 82(12) 6039-6044.

Laboratory

Associate Professor: Kentaro Ibuki, D.V.M., Ph.D.

Tel : +81-75-751-4178
FAX : +81-75-751-3909
e-mail : ibuki.kentaro.7s@kyoto-u.ac.jp