Koji Eto, M.D., Ph.D. Professor
We engage in basic research on the generation of blood cells including hematopoietic stem cells (HSCs), platelets, or red blood cells in vitro from human iPS cells toward clinical applications.
Research and EducationThe research program aims at the development of safe and stable blood supply for transfusion independently of blood donation and of gene therapy using established HSCs with an appropriate validation. Alternative program aims at the elucidation of molecular mechanisms how self-renewal of megakaryocyte progenitor cells, precursors of platelets, are maintained through epigenetic modification and how symmetrical or asymmetrical self-renewal of HSCs are retained by extracellular circumstances, particularly mediated through adhesion/integrin signaling, which may be useful for efficient induction of human iPS cell-derived megakaryocytes and platelets or transplantable HSCs.
Safe and stable blood supply for transfusion independently of blood donation.
Efficient induction of human ESC/iPSC-derived megakaryocytes and platelets.
Recent Publications1． Nakamura S, Takayama N, Hirata S, Seo H, Endo H, Ochi K, Fujita KI, Koike T, Harimoto KI, Watanabe A, Okita K, Takahashi N, Sawaguchi A, Yamanaka S, Nakauchi H, Nishimura S, Eto K. Expandable megakaryocyte cell lines enable clinically-aplicable generation of platelets from human induced pluripotent stem cells. Cell Stem Cell. 2014 Feb 12；14(4):535-548. doi: 10.1016/j.stem.2014.01.011.
2．Hirose S, Takayama N, Nakamura S, Nagasawa K, Ochi K, Hirata S, Yamazaki S, Yamaguchi T, Otsu M, Sano S, Takahashi N, Sawaguchi A, Ito M, Kato T, Nakauchi H, Eto K. Immortalization of erythroblasts by c-MYC and BCL-XL enables large-scale erythrocyte production from human pluripotent stem cells. Stem Cell Reports. 2013 Dec 5;1(6):499-508. doi: 10.1016/j.stemcr.2013.10.010. eCollection 2013.
3. Hirata S, Takayama N, Jono-Ohnishi R, Endo H, Nakamura S, Dohda T, Nishi M, Hamazaki Y, Ishii EI, Kaneko S, Otsu M, Nakauchi H, Kunishima S, Eto K. Congenital amegakaryocytic thrombocytopenia iPS cells exhibit defective MPL-mediated signaling. J Clin Invest. 2013 Sep 3;123(9):3802-14. doi: 10.1172/JCI64721. Epub 2013 Aug 1.
4. Nishimura T, Kaneko S, Kawana-Tachikawa A, Tajima Y, Goto H, Zhu D, Nakayama-Hosoya K, Iriguchi S, Uemura Y, Shimizu T, Takayama N, Yamada D, Nishimura K, Ohtaka M, Watanabe N, Takahashi S, Iwamoto A, Koseki H, Nakanishi M, Eto K, Nakauchi H. Generation of rejuvenated antigen-specific T cells by reprogramming to pluripotency and redifferentiation. Cell Stem Cell. 2013 Jan 3;12(1):114-26. doi: 10.1016/j.stem.2012.11.002.
5. Takayama N, Nishimura S, Nakamura S, Shimizu T, Ohnishi R, Endo H, Yamaguchi T, Otsu M, Nishimura K, Nakanishi M, Sawaguchi A, Nagai R, Takahashi K, Yamanaka S, Nakauchi H, Eto K. Transient activation of c-MYC expression is critical for efficient platelet generation from human induced pluripotent stem cells. J Exp Med 207, 2817-30.
Stem Cell Biology and ApplicationProfessor Koji Eto
Assistant Professor Naoshi Sugimoto
Researcher Sou Nakamura
Researcher Hiroshi Endo