Pathogenic Microbiology

kao Mitsuaki Nishibuchi,Ph.D.   btn

Infection is the war between the pathogen and its host. It is important to understand the nature of both to prevent and treat the infection. We study bacterial pathogens. The habitat of most bacterial pathogens is not humans but the natural environment. Knowledge and techniques of various fields are needed to understand how the pathogenic bacteria distinguish the natural environment and humans and how they act in the different environments. Some infections are spread across the international borders and international collaboration among the researchers is imperative to study the epidemiology.

Research and Education

We perform research:
– on the enteric infections caused by the pathogens such as Vibrio cholerae, Vibrio parahaemolyticus, and diarrheagenic Escherichia coli that are important in Southeast Asia.
– on the pathogens from the environment and the patients in collaboration with the workers in Asia.
– on the isolated bacterium for the characteristics (the serotype, phage type, genotype, etc.) unique to the environment.
– whether a particular strain in the environment is responsible for the local infection or the infection is spread across the international borders.
– how the pathogenic strains distinguish the signals in the natural environment and the host and then express their virulence properties
– , through laboratory work (experiments) and field work (sampling and hearing), and by using the techniques associated with the environmental microbiology, clinical microbiology, molecular genetics, physiology, and immunology.
We also cooperate with educational programs of various graduate schools (Public Health, Asian and African Studies, The Inter-Graduate School Program (Global Survivability Studies Program or GSS).

r-105-1 Graduate students of our group teach specialized laboratory techniques to young Asian scientists and Kyoto University in our laboratory as a part of a field study program of The Inter-Graduate School Program (GSS)(December, 2012)

r-105-2 Interview on food poisoning cases at Ministry of Health, Singapore as a part of a field study program of The Inter-Graduate School Program (GSS)(March, 2013)

Recent Publications

  1. Escalante-Maldonado, O., et al. 2015. Improvement of the quantitation method for the tdh+ Vibrio parahaemolyticus in molluscan shellfish based on most-probable- number, immunomagnetic separation, and loop-mediated isothermal amplification. Front. Microbiol. doi: 10.3389/fmicb.2015.00270.
  2. Yingkajorn M., et al. 2014. Vibrio parahaemolyticus and its specific bacteriophages as an indicator in cockles (Anadara granosa) for the risk of V. parahaemolyticus infection in southern Thailand. Microbil. Ecol. 67(4): 849-856
  3. Sermwittayawong N., et al. 2012. Human Plasmodium knowlesi infection in Ranong province, southwestern border of Thailand. Malaria Journal 11:36 (
  4. Loo, Y. Y. et al. 2012. Synthesis of silver nanoparticles by using tea leaf extract from Camellia sinensis. Int. J. Nanomedicine 7:4263-4267.
  5. Chen, Y., et al. 2011. Comparative genomic analysis of Vibrio parahaemolyticus: serotype conversion and virulence. BMC Genomics 12(1): 294.


Professor: Mitsuaki Nishibuchi
Specific Assistant Professor: Kayali Ahamad Yaman
TEL:075-753-7367, 7319; 075-761-2700
FAX:075-753-7319; 075-761-2701