Viral Pathogenesis

小柳 Yoshio Koyanagi, M.D., Ph.D. Professor  btn

From retrovirus researches it has been achieved the discoveries of reverse transcriptase and retroviral oncogenes. It is clear that these discoveries provided strong advances on Virology and Cell Biology. Then, it has been well known that ‘‘retroviruses can cause some human diseases’’ after the discovery of human immunodeficiency virus (HIV). We now know the importance of retrovirus research. Therefore, we believe that our efforts will contribute to Medical as well as Life Sciences.

Research and Education

Research projects have been arranged.

1) Replication of viral genome
Virus infects cell and replicates. Viral genome moves from virion-produced cell to adjacent naive cells. This is a most significant characteristic of virus. The mechanism of this infection event is a primary theme. The genome movement is not restricted in cell to cell infection. Inside cells, viral genomes or/and viral proteins move. Virus uses cellular trafficking machinery. We wish to learn the mechanism.

2) Regulation of HIV replication by host molecules
Virus cannot replicate without cells. Since it has been finding many factors on HIV replication, we wish to learn the mechanism of HIV replication from aspect of Immunology and Virology.

3) Mechanism of HIV pathogenesis
HIV causes immunodeficiency in human. The mechanism of the immunodeficiency remains unclear. It has been analyzing how the immunodeficiency occurs using in vitro-cell culture models and in vivo-animal models. We developed a mouse system that human immune system is transplanted in SCID mouse and in this human-chimera mouse abundant CD4 cell killing can be reproduced with HIV infection.



ウイルス研感染病態写真Laboratory members

Recent Publications

  1. Ebina H, Kanemura Y, Misawa N, Sakuma T, Kobayashi T, Yamamoto T, Koyanagi Y. A high excision potential of TALENs for integrated DNA of HIV-based lentiviral vector. (2015) PLoS One, e0120047.
  2. Yoshikawa R, Izumi T, Yamada E, Nakano Y, Misawa N, Ren F,Carpenter M A, Ikeda T, Münk C, Harris RS, Miyazawa T, Koyanagi Y and Sato K. (2015) A naturally occurring domestic cat APOBEC3 variant confers resistance to FIV infection. J Virol, 90:474-85.
  3. Iwami S, Takeuchi JS, Nakaoka S, Mammano F, Clavel F, Inaba H, Kobayashi T, Misawa N, Aihara K, Koyanagi Y, Sato K. (2015) Cell-to-cell infection by HIV contributes over half of virus infection. eLife 4:e08150.
  4. Sato K, Takeuchi SJ, Misawa N, Izumi T, Kobayashi T, Kimura Y, Iwami S, Takaori-Kondo A, Hu WS, Aihara K, Ito M, An DS, Pathak VK, Koyanagi Y. (2014) APOBECD and APOBECF potently promote HIV-1 diversification and evolution in humanized mice. PLoS Pathog, 10:e1004453.
  5. Sato, K, Misawa N, Iwami S, Satou Y, Matsuoka M, Ishizaka Y, Ito M, Aihara K, An DS, Koyanagi Y. (2013) HIV-1 Vpr accelerates viral replication during acute infection by exploitation of proliferating CD4+ T cells in vivo. PLoS Pathog, 9:e1003812.


Laboratory of Viral Pathogenesis, Research Center for Human Retroviruses
Institute for Virus Research
Professor : Yoshio Koyanagi
Associate professor : Kei Sato
TEL : +81-75-751-4813
FAX : +81-75-751-4812
e-mail :