Development and Differentiation

kao Norio Nakatsuji, Ph.D Professor btn

Our research group has been working on development and differentiation of embryonic stem cells and germ cells in mammals. In particular, we have established mouse, cynomolgus monkey, and human embryonic stem (ES) cell lines, and we have been carrying out various aspects of basic and application research using pluripotent stem cells.

Research and Education

We are carrying out multidisciplinary research of germ lineage cells and pluripotent stem cells (ES and iPS cells). One example is neurodegenerative disease model cells produced by genetic modification of pluripotent stem cell lines and differentiation into relevant cells. Also, we are aiming at control of stem cells by small molecules and functional materials to control growth and differentiation, such as into cardiomyocytes, of human ES/iPS cells in collaboration with chemical biology and nano/meso/micro-engineering groups.

r-087-1 1. Human ES cell colony

r-087-2

2. Development of differentiation method from human ES/iPS cells using small chemical compounds


Recent Publications

1. International Stem Cell Initiative. Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage. Nat Biotechnol. 29:1132-44 (2011).
2. Tanaka, T., Hosokawa, M., Vagin, V. V., Reute,r M., Hayashi, E., Mochizuki, A. L., Kitamura, K., Yamanaka, H., Kondo,h G., Okawa, K., Kuramochi-Miyagawa, S., Nakano, T., Sachidanandam, R., Hannon, G. J., Pillai, R. S., Nakatsuji, N., Chuma, S. Tudor domain containing 7 (Tdrd7) is essential for dynamic ribonucleoprotein (RNP) remodeling of chromatoid bodies during spermatogenesis. Proc Natl Acad Sci U S A. 108:10579-84 (2011).
3. Wada, T., Goparaju, S. K., Tooi, N., Inoue, H. Takahashi, R., Nakatsuji, N. and Aiba, K. Amyotrophic lateral sclerosis model derived from human embryonic stem cells overexpressing mutant superoxide dismutase 1. Stem Cells Translational Medicine 1: 396–402 (2012).
4. Minami, I., Yamada, K., Otsuji, T. G., Yamamoto, T., Shen, Y., Otsuka, S., Kadota, S., Morone, N., Barve, M., Asai, Y., Tenkova-Heuser, T., Heuser, J. E., Uesugi, M., Aiba, K. and Nakatsuji, N. A Small Molecule that Promotes Cardiac Differentiation of Human Pluripotent Stem Cells under Defined, Cytokine- and Xeno-free Conditions. Cell Reports 2(5): 1448–1460 (2012).
5. Miyazaki, T., Futaki, S., Suemori, H., Taniguchi, Y., Yamada, M., Kawasaki, M., Hayashi, M., Kumagai, H., Nakatsuji, N., Sekiguchi, K. and Kawase, E. Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells. Nature Communications 3: article# 1236 (2012). doi:10.1038/ncomms2231

Laboratory

Professor: Norio Nakatsuji
Associate Professor: Shinichiro Chuma
TEL : +81-75-751-3821
FAX : +81-75-751-3890
e-mail : nnakatsu@frontier.kyoto-u.ac.jp
URL : http://www.frontier.kyoto-u.ac.jp/rc01/index-j.htm