Pathology and Biology of Diseases

Michiyuki Matsuda, M.D., Ph.D. Professor btn

Pathology is a school of medicine, which attempts to establish the basis of medical treatment by examining the difference between healthy and diseased tissues and thereby revealing the cause of diseases. Traditionally, we have been using macroscopic and microscopic methods with the diseased organs and tissues. However, we now adds not only new methodologies of molecular biology and genetics, but also state-of-art fluorescent imaging techniques, computer simulation, and mathematical modeling to challenge the questions of cancer and many other diseases.

Research and Education

The principal concept of our laboratory is “Cancer research based on microscopic imaging”. In 2001 we for the first time developed a probe for the activity of the Ras oncogene product, and thereafter we have developed nearly fifty probes for many signaling molecules. With several sophisticated microscopes such as multi-photon excitation microscopes, we are attempting to understand the disease by live-imaging of the very moment when diseases occur. In parallel, based on the multi-dimensional imaging data that contain the spatio-temporal information of molecular activities, we are building mathematical models of oncogene networks. Thus, we are moving forward from the classical pathology based on the observation of diseased tissues to pathology that live-images the disease in situ and that foresees the disease by simulation. Students in our laboratory come from schools of medicine, biology, engineering, physics etc., and study together on molecular biology, pathology, imaging technologies, systems biology etc. We speak English in the laboratory meetings to foster students who seek their future in the world.

Fig.1-RGBERK activity map in a mouse

Laboratory members

Recent Publications

  1. Konagaya Y, Terai K, Hirao Y, Takakura K, Imajo M, Kamioka Y, Sasaoka N, Kakizuka A, Sumiyama K, Asano T, & Matsuda M. A Highly Sensitive FRET Biosensor for AMPK Exhibits Heterogeneous AMPK Responses among Cells and Organs. Cell Rep. 21, 2628-2638, (2017).
  2. Uda Y, Kohchi T, Matsuda M & Aoki K. An efficient synthesis of phycocyanobilin in mammalian cells for optogenetic control of cell signalings. Proc Natl Acad Sci USA 114, 11962-11967, (2017).
  3. Aoki K, Kondo Y, Naoki H, Hiratsuka T, Itoh RE, & Matsuda M. Propagating wave of ERK activation orients collective cell migration. Dev Cell 43, 305-317, (2017).
  4. Yamauchi F, Kamioka Y, Yano T, & Matsuda M. In Vivo FRET Imaging of Tumor Endothelial Cells Highlights a Role of Low PKA Activity in Vascular Hyperpermeability. Cancer Res. 76, 5266-76, (2016).
  5. Hiratsuka T, Fujita Y, Naoki H, Aoki K, Kamioka Y, & Matsuda M. Intercellular propagation of extracellular signal-regulated kinase activation revealed by in vivo imaging of mouse skin. eLife. 4, e05178 (2015).

Pathology and Biology of Diseases

Pathology and Biology of Diseases
Professor: Michiyuki Matsuda
Assistant Professor: Tsuyoshi Hirashima
TEL:+81-75-753-4697 (office), 9450 (laboratory)