Pathology and Biology of Diseases

Michiyuki Matsuda, M.D., Ph.D. Professor btn

Pathology is a school of medicine, which attempts to establish the basis of medical treatment by examining the difference between healthy and diseased tissues and thereby revealing the cause of diseases. Traditionally, we have been using macroscopic and microscopic methods with the diseased organs and tissues. However, we now adds not only new methodologies of molecular biology and genetics, but also state-of-art fluorescent imaging techniques, computer simulation, and mathematical modeling to challenge the questions of cancer and many other diseases.

Research and Education

The principal concept of our laboratory is “Cancer research based on microscopic imaging”. In 2001 we for the first time developed a probe for the activity of the Ras oncogene product, and thereafter we have developed nearly fifty probes for many signaling molecules. With several sophisticated microscopes such as multi-photon excitation microscopes, we are attempting to understand the disease by live-imaging of the very moment when diseases occur. In parallel, based on the multi-dimensional imaging data that contain the spatio-temporal information of molecular activities, we are building mathematical models of oncogene networks. Thus, we are moving forward from the classical pathology based on the observation of diseased tissues to pathology that live-images the disease in situ and that foresees the disease by simulation. Students in our laboratory come from schools of medicine, biology, engineering, physics etc., and study together on molecular biology, pathology, imaging technologies, systems biology etc. We speak English in the laboratory meetings to foster students who seek their future in the world.

Fig.1-RGBERK activity map in a mouse

Laboratory members

Recent Publications

  1. Hiratsuka T, Sano T, Kato T, Komatsu N, Imajo M, Kamioka Y, Sumiyama K, Banno F, Miyata T, & Matsuda M. Live imaging of ERK and PKA activities during thrombus formation in mice expressing biosensors based on Förster resonance energy transfer. J. Thromb. Haemost., in press.
  2. Yamauchi F, Kamioka Y, Yano T, & Matsuda M. In Vivo FRET Imaging of Tumor Endothelial Cells Highlights a Role of Low PKA Activity in Vascular Hyperpermeability. Cancer Res. 76, 5266-76, (2016).
  3. Hiratsuka T, Fujita Y, Naoki H, Aoki K, Kamioka Y, & Matsuda M. Intercellular propagation of extracellular signal-regulated kinase activation revealed by in vivo imaging of mouse skin. eLife. 4, e05178 (2015).
  4. Mizuno R, Kamioka Y, Kabashima K, Imajo M, Sumiyama K, Nakasho E, Ito T, Hamazaki Y, Okuchi Y, Sakai Y, Kiyokawa E & Matsuda M. In vivo FRET imaging of activities of PKA and ERK during neutrophil recruitment to inflamed intestines. J. Exp. Med., 211, 1123-1136, (2014).
  5. Aoki K, Kumagai Y, Sakurai A, Komatsu N, Fujita Y, Shionyu C & Matsuda, M. Stochastic ERK activity pulses induced by noise and cell-to-cell propagation regulate density-dependent proliferation. Mol. Cell 52, 529-540, (2013).

Pathology and Biology of Diseases

Pathology and Biology of Diseases
Professor: Michiyuki Matsuda
Assistant Professor: Tsuyoshi Hirashima
TEL:+81-75-753-4697 (office), 9450 (laboratory)