It is printed in the A4 size.To the laboratory site

 - Basic Medicine (Core Departments) - Basic Pathology
Pathology and Biology of Diseases
Pathology is the school of science that takes advantage of any methodology for the understanding of the diseases. Our laboratory has been achieving many feats in the experimental oncology such as the discovery of Fujinami Sarcoma Virus, which was named after the founder of our department, Professor Akira Fujinami. We succeed this tradition and attempt to decipher the enigma of cancer development by using variety of techniques, which includes Fluorescence resonance energy transfer (FRET) imaging, two-photon excitation fluorescence microscopy, and computer simulation.

  Michiyuki Matsuda, M.D., Ph.D.
Research and Education
The basic concept of this department is the cancer research based on the morphological methods. We have been developing FRET probes for the monitoring of oncogene products in living cells. The first successful image of Ras activation reported in 2001 can be found in many textbooks of cell biology. To date this research group has been developed more than 20 probes for G proteins, kinases, and phospholipids. These probes are used to visualize the growth signal transduction cascades and to collect parameters that determine the property of this signaling system. Such parameters are invaluable in that they provide the foundation of the virtual cancer cells. Students in our department come from schools of medicine, biology, physics, and so on. They are enthusiastically learning not only modern molecular biology and pathology, but also bioimaging and system biology. We pay particular focus on the teaching of English communication. Many students present their data in the meetings abroad.

Pathology and Biology of Diseases
Professor Michiyuki Matsuda

Takuya Hiratsuka
TEL +81-75-753-4697(office), 9450 (laboratory)
FAX +81-75-753-4655
Live imaging of the skin of a transgenic mouse
Live imaging of Protein kinase A activity in the intestine
Simulation model of EGF receptor signaling
Video image of glioma cell invasion into the brain
Recent Publications
1. Kamioka,Y, Sumiyama,K., Mizuno,R., Sakai,Y., Hirata,E., Kiyokawa,E., & Matsuda.M. Live imaging of protein kinase activities in transgenic mice expressing FRET biosensors. Cell Struct.Funct. 37, 65-73 (2012).
2. Hirata,E. Yukinaga,H., Kamioka,Y., Arakawa,Y., Miyamoto,S., Okada,T., Sahai,E., & Matsuda,M. In vivo fluorescence resonance energy transfer imaging reveals differential activation of Rho-family GTPases in glioblastoma cell invasion. J. Cell Sci. 125, 858-868 (2011).
3. Aoki,K., Yamada,M., Kunida,K., Yasuda,S. & Matsuda,M. Processive phosphorylation of ERK MAP kinase in mammalian cells. Proc Natl Acad Sci U S A 12675-12680 (2011).
4. Hara,S., Kiyokawa,E., Iemura,S.I., Natsume,T., Wassmer,T., Cullen,P.J., Hiai,H. & Matsuda,M. The DHR1 domain of DOCK180 binds to SNX5 and regulates cation-independent mannose 6-phosphate receptor transport. Mol. Biol. Cell 19, 3823-3835 (2008).
5. Kitano,M., Nakaya,M., Nakamura,T., Nagata,S. & Matsuda,M. Imaging of Rab5 activity identifies essential regulators for phagosome maturation. Nature 453, 241-245 (2008).